Gel for use in polyacrylamide gel electrophoresis and electrophoresis device using said gel
US-2016334364-A1 · Nov 17, 2016 · US
US9028664B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9028664-B2 |
| Application number | US-201013390352-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 18, 2010 |
| Priority date | Aug 18, 2009 |
| Publication date | May 12, 2015 |
| Grant date | May 12, 2015 |
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A device for isoelectric focusing. The device comprises a focusing container configured to contain an electrolyte solution and having a longitudinal axis and at least one electrolysis unit mounted in a close proximity to the longitudinal axis. Each electrolysis unit injects an ion flow into the focusing container so as to create a pH gradient having a plurality of steps in the electrolyte solution, along the longitudinal axis. Each step has a substantially uniform pH level and the pH gradient is defined by at least one pH ramp between every two sequential steps of the plurality of steps.
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What is claimed is: 1. A method for isoelectric focusing, comprising: providing a focusing container having a channel along a longitudinal axis, said channel is configured to contain an electrolyte solution, said focusing container having a plurality of electrolysis units, distributed along said longitudinal axis, each having at least one electrode; and receiving an input defining any of a plurality of desired pH gradients having a plurality of steps in said electrolyte solution…
Physics · mapped topic
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