Organisms for the production of 1,3-butanediol

Patent metadata

Field	Value
Publication number	US-9017983-B2
Application number	US-77211410-A
Country	US
Kind code	B2
Filing date	Apr 30, 2010
Priority date	Apr 30, 2009
Publication date	Apr 28, 2015
Grant date	Apr 28, 2015

Abstract

A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

First claim

What is claimed is:

1. A non-naturally occurring microbial organism, comprising a set of enzymes that convert acetoacetyl-CoA to 1,3-butanediol, wherein said organism comprises at least two exogenous nucleic acids each encoding an enzyme of the set, which are expressed in a sufficient amount to produce the 1,3-butanediol, wherein the set of enzymes comprises:
an acetoacetyl-CoA reductase (ketone reducing) that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA; and
(i) a 3-hydroxybutyryl-CoA reductase (aldehyde forming) that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde; or
(ii) a 3-hydroxybutyraldehyde reductase that converts 3-hydroxybutyraldehyde to 1,3-butanediol;
and wherein said microbial organism produces 1,3-butanediol.

2. The non-naturally occurring microbial organism of claim 1 , wherein the microbial organism comprises three exogenous nucleic acids, each encoding an enzyme of the set.

3. The non-naturally occurring microbial organism of claim 1 , wherein at least one exogenous nucleic acid is a heterologous nucleic acid.

4. The non-naturally occurring microbial organism of claim 1 , wherein the at least two exogenous nucleic acids encode the acetoacetyl-CoA reductase (ketone reducing) and the 3-hydroxybutyryl-CoA reductase (aldehyde forming).

5. The non-naturally occurring microbial organism of claim 1 , wherein the at least two exogenous nucleic acids encode the acetoacetyl-CoA reductase (ketone reducing) and the 3-hydroxybutyraldehyde reductase.

Assignees

• Burgard Anthony P
• Burk Mark J
• Osterhout Robin E
• Pharkya Priti
• Genomatica Inc

Inventors

• Burgard Anthony P
• Burk Mark J
• Osterhout Robin E
• Pharkya Priti

Classifications

• C12N9/88

  Lyases (4.) · CPC title

• C12N9/0008

  acting on the aldehyde or oxo group of donors (1.2) · CPC title

• C12Y402/01

  Hydro-lyases (4.2.1) · CPC title

• C12N15/52 Primary

  Genes encoding for enzymes or proenzymes · CPC title

• C12N1/38

  Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title