Soybean event MON89788 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-9017947-B2
Application number	US-201113214081-A
Country	US
Kind code	B2
Filing date	Aug 19, 2011
Priority date	May 27, 2005
Publication date	Apr 28, 2015
Grant date	Apr 28, 2015

Abstract

The present invention provides for soybean plant and seed comprising transformation event MON89788 and DNA molecules unique to these events. The invention also provides methods for detecting the presence of these DNA molecules in a sample.

First claim

What is claimed:

1. A method of detecting the presence of DNA corresponding to the soybean event MON89788 in a sample, the method comprising:
(a) contacting a sample comprising soybean DNA with a primer set, wherein a first DNA molecule of the primer set comprises at least 11 contiguous nucleotides of SEQ ID NO:3, SEQ ID NO:4 or a full complement thereof to function when used in a nucleic acid amplification reaction with DNA from soybean event MON89788 to produce a diagnostic amplicon for soybean event MON89788, wherein the diagnostic amplicon comprises SEQ ID NO:1, SEQ ID NO:2, or a full complement thereof; and
(b) performing a nucleic acid amplification reaction, thereby producing the diagnostic amplicon; and
(c) detecting the diagnostic amplicon.

2. A method of detecting the presence of a nucleic acid corresponding to event MON89788 in a sample, the method comprising:
(a) obtaining a sample of soybean DNA;
(b) contacting the sample with a DNA probe comprising a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:3, SEQ ID NO:4, or a full complement thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:2 and does not hybridize under stringent hybridization conditions with a DNA molecule not comprising SEQ ID NO:1 or SEQ ID NO:2;
(c) subjecting the sample and DNA probe to stringent hybridization conditions; and
(d) detecting hybridization of the DNA probe to DNA in the sample, wherein detection of hybridization indicates the presence of a DNA molecule from a transgenic soybean plant comprising event MON89788.

3. The method of claim 2 , wherein the DNA probe comprises SEQ ID NO:1, SEQ ID NO:2, or a full complement thereof.

4. The method of claim 1 , wherein the diagnostic amplicon comprises SEQ ID NO:1.

5. The method of claim 1 , wherein the diagnostic amplicon comprises SEQ ID NO:2.

Assignees

• Malven Marianne
• Rinehart Jennifer
• Taylor Nancy
• Dickinson Ellen
• Monsanto Technology Llc

Inventors

• Malven Marianne
• Rinehart Jennifer
• Taylor Nancy
• Dickinson Ellen

Classifications

• C12N15/821

  Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers · CPC title

• C12N15/8275 Primary

  Glyphosate · CPC title

• A01N57/20

  containing acyclic or cycloaliphatic radicals · CPC title

• C12N15/8286

  for insect resistance · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title