Systems and methods for monitoring the amplification of DNA

Patent metadata

Field	Value
Publication number	US-9017946-B2
Application number	US-14422308-A
Country	US
Kind code	B2
Filing date	Jun 23, 2008
Priority date	Jun 23, 2008
Publication date	Apr 28, 2015
Grant date	Apr 28, 2015

Abstract

A system and method for amplifying and detecting nucleic acids are disclosed. In one embodiment, the system includes: a microfluidic device comprising a channel for receiving a sample of solution containing real-time PCR reagents; a temperature control system configured to cycle the temperature of the sample; an excitation source for illuminating the sample; a fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel; and a detector configured to detect emissions exiting the proximal end of the optical fiber.

First claim

What is claimed is:

1. A system for performing real-time PCR, comprising:
a microfluidic device comprising a microfluidic chip comprising at least one channel for receiving a sample of solution containing real-time PCR reagents;
a temperature control system configured to cycle the temperature of the sample; and
an imaging system for detecting emissions from the sample, wherein the imaging system comprises:
an excitation source for illuminating the sample,
at least two fiber…

Assignees

• Hasson Kenton C
• Canon Us Life Sciences Inc

Inventors

• Hasson Kenton C

Classifications

• C12Q1/686 Primary

  Chemistry & Metallurgy · mapped topic