Method for sequencing a polynucleotide template

US9017945B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9017945-B2
Application numberUS-201213556053-A
CountryUS
Kind codeB2
Filing dateJul 23, 2012
Priority dateJul 20, 2005
Publication dateApr 28, 2015
Grant dateApr 28, 2015

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Abstract

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The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.

First claim

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The invention claimed is: 1. A method for pairwise sequencing of first and second strands of target polynucleotides, wherein the first and second strands of each target polynucleotide have complementary sequences, the method comprising: (a) forming a plurality of nucleic acid colonies by solid phase nucleic acid amplification, wherein each of the nucleic acid colonies comprises multiple copies of a first strand and a complementary second strand formed by amplification of a single…

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What does patent US9017945B2 cover?
The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
Who is the assignee on this patent?
Smith Geoffrey Paul, Illumina Cambridge Ltd
What technology area does this patent fall under?
Primary CPC classification C12Q1/6874. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Apr 28 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).