Compositions and methods for accurately identifying mutations
US-2024409996-A1 · Dec 12, 2024 · US
US9017945B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9017945-B2 |
| Application number | US-201213556053-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 23, 2012 |
| Priority date | Jul 20, 2005 |
| Publication date | Apr 28, 2015 |
| Grant date | Apr 28, 2015 |
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The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
Opening claim text (preview).
The invention claimed is: 1. A method for pairwise sequencing of first and second strands of target polynucleotides, wherein the first and second strands of each target polynucleotide have complementary sequences, the method comprising: (a) forming a plurality of nucleic acid colonies by solid phase nucleic acid amplification, wherein each of the nucleic acid colonies comprises multiple copies of a first strand and a complementary second strand formed by amplification of a single…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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