Designer circuit controlling diet-induced obesity
US-2015368666-A1 · Dec 24, 2015 · US
US9017657B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9017657-B2 |
| Application number | US-32002609-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 15, 2009 |
| Priority date | Jul 16, 2008 |
| Publication date | Apr 28, 2015 |
| Grant date | Apr 28, 2015 |
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The invention relates to a cell culture method, particularly to a co-culture method for human mesenchymal stem cells and target animal cells, in order to solve the problem that animal cells are not easy to survive alone upon culturing. The invention also provides a method for using a stem cell conditioned medium to culture animal cells. The invention also provides a method to induce the transformation of human fetal islet-like cell clusters from human stem cells and its application thereof.
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What is claimed is: 1. A method to induce transformation of human islet cell cluster from human mesenchymal stem cells, comprising, (i) isolating human umbilical cord mesenchymal stem cells (HUMSCs) from human umbilical cord; (ii) culturing the isolated HUMSCs in a medium containing serum; (iii) culturing the cultured HUMSCs in mammalian neuronal cell conditioned medium (NCM) containing cytosine arabinoside (AraC) to produce nestin-positive cells; (iv) culturing the nestin-p…
Human Necessities · mapped topic
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