Islet cell cluster produced from human umbilical cord mesenchymal stem cells

US9017657B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9017657-B2
Application numberUS-32002609-A
CountryUS
Kind codeB2
Filing dateJan 15, 2009
Priority dateJul 16, 2008
Publication dateApr 28, 2015
Grant dateApr 28, 2015

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Abstract

Official abstract text for this publication.

The invention relates to a cell culture method, particularly to a co-culture method for human mesenchymal stem cells and target animal cells, in order to solve the problem that animal cells are not easy to survive alone upon culturing. The invention also provides a method for using a stem cell conditioned medium to culture animal cells. The invention also provides a method to induce the transformation of human fetal islet-like cell clusters from human stem cells and its application thereof.

First claim

Opening claim text (preview).

What is claimed is: 1. A method to induce transformation of human islet cell cluster from human mesenchymal stem cells, comprising, (i) isolating human umbilical cord mesenchymal stem cells (HUMSCs) from human umbilical cord; (ii) culturing the isolated HUMSCs in a medium containing serum; (iii) culturing the cultured HUMSCs in mammalian neuronal cell conditioned medium (NCM) containing cytosine arabinoside (AraC) to produce nestin-positive cells; (iv) culturing the nestin-p…

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  • A61K38/27Primary

    Human Necessities · mapped topic

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What does patent US9017657B2 cover?
The invention relates to a cell culture method, particularly to a co-culture method for human mesenchymal stem cells and target animal cells, in order to solve the problem that animal cells are not easy to survive alone upon culturing. The invention also provides a method for using a stem cell conditioned medium to culture animal cells. The invention also provides a method to induce the transfo…
Who is the assignee on this patent?
Liu Shing-Hwa, Chao Kuo-Ching, Chao Kuo-Fang, and 1 more
What technology area does this patent fall under?
Primary CPC classification A61K38/27. Mapped technology areas include Human Necessities.
When was this patent published?
Publication date Tue Apr 28 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).