Methods for simultaneous quantification of thyroid hormones and metabolites thereof by mass spectrometry

Patent metadata

Field	Value
Publication number	US-9012835-B2
Application number	US-201113881602-A
Country	US
Kind code	B2
Filing date	Nov 7, 2011
Priority date	Nov 8, 2010
Publication date	Apr 21, 2015
Grant date	Apr 21, 2015

Abstract

The invention provides methods for simultaneously detecting or simultaneously quantifying any combination of thyroxine (T 4 ), triiodothyronine (T 3 ), 3,3′-diiodo-L-thyronine (3,3′-T 2 ), 3-iodothyronamine (T 1 AM), and, optionally, reverse T 3 (rT 3 ) in a sample obtained from a human. The method involves a simple, sensitive, accurate, and specific isotope dilution tandem mass spectrometry method for the simultaneous quantification of any combination of T 4 , T 3 , 3,3′-T 2 , T 1 AM, and, optionally, rT 3 in a sample obtained from a human, e.g., in human plasma or serum samples. This assay is far more sensitive than previously described assays for thyronamines and allows quantitation of T 1 AM in human plasma or serum, including from healthy controls.

First claim

What is claimed is:

1. A method of simultaneously assaying thyroxine (T 4 ), triiodothyronine (T 3 ), 3,3′-diiodo-L-thyronine (3,3′-T 2 ), and 3-iodothyronamine (T 1 AM), comprising:
a) providing a test sample, wherein the test sample comprises T 4 , T 3 , 3,3′-T 2 , and T 1 AM;
b) providing a calibration sample, wherein the calibration sample comprises known quantities of reference T 4 , T 3 , 3,3′-T 2 , and T 1 AM;
c) combining the test sample with the calibration sample;…

Assignees

• Soldin Steven J
• Soldin Offie P
• Univ Georgetown

Inventors

• Soldin Steven J
• Soldin Offie P

Classifications

• G01N30/7233

  Physics · mapped topic

• H01J49/26

  Electricity · mapped topic

• G01N33/6848 Primary

  Physics · mapped topic

• G01N33/487 Primary

  Physics · mapped topic

• G01N33/492

  Physics · mapped topic