In situ heat induced antigen recovery and staining method
US-2016370390-A1 · Dec 22, 2016 · US
Hydrogel nanoparticle based immunoassay
US9012240B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012240-B2 |
| Application number | US-200913061507-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 26, 2009 |
| Priority date | Aug 26, 2008 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Abstract
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An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the immunoassay device. A preferred embodiment is coreshell bait containing capture nanoparticles which perform three functions in one step, in solution: a) molecular size sieving, b) target analyte sequestration and concentration, and c) protection from degradation. The polymeric matrix of the capture particles may be made of co-polymeric materials having a structural monomer and an affinity monomer, the affinity monomer having properties that attract the analyte to the capture particle. This device is useful for point of care diagnostic assays for biomedical applications and as field deployable assays for environmental, pathogen and chemical or biological threat identification.
First claim
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We claim: 1. A method of analyte enrichment in an immunoassay, the method comprising: providing an immunoassay device comprising a wicking matrix upstream of a porous matrix and an analyte binding region downstream of said porous matrix; adding a sample fluid containing the analyte to porous hydrogel nanoparticles and allowing the analyte to sequester within the porous hydrogel nanoparticles, wherein said porous hydrogel nanoparticles i) range in size from 1 nm to 100 μm; ii) co…
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- G01N33/521Primary
Physics · mapped topic
Physics · mapped topic
Physics · mapped topic
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- What does patent US9012240B2 cover?
- An immunoassay device incorporating porous polymeric capture nanoparticles within either the sample collection vessel or pre-impregnated into a porous substratum within fluid flow path of the analytical device is presented. This incorporation of capture particles within the immunoassay device improves sensitivity while removing the requirement for pre-processing of samples prior to loading the …
- Who is the assignee on this patent?
- Liotta Lance A, Luchini Alessandra, Petricoin Emanuel F, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification G01N33/521. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).