Stimulation-responsive material and cell culture container made thereof
US-2015344831-A1 · Dec 3, 2015 · US
US9012193B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012193-B2 |
| Application number | US-201313777949-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 26, 2013 |
| Priority date | Oct 10, 2005 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micromechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.
Opening claim text (preview).
What is claimed: 1. A micro-patterned plate for patterning of biological materials of interest at specific sites on a plate, the materials of interest comprising cells, tissues, DNA or living organisms, comprising a body, a plurality of elevated sites attached to and extending up from a surface of the body which come into contact with a liquid solution upon submersion of the plate in the liquid solution, wherein the nature of the attachment of the plurality of elevated sites to…
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