Library preparation methods and compositions and uses therefor
US-12065643-B2 · Aug 20, 2024 · US
End modification to prevent over-representation of fragments
US9012184B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012184-B2 |
| Application number | US-22376107-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 7, 2007 |
| Priority date | Feb 8, 2006 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Abstract
Official abstract text for this publication.
The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from when the 5′ and 3′ modified library is generated is greatly reduced or prevented.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of reducing representation of the ends of a primary polynucleotide sequence in a library of fragments generated from said primary polynucleotide sequence, the method comprising: a) performing an amplification reaction with a modified forward primer and a modified reverse primer to introduce a modification at or near each 5′ terminus of a double-stranded primary polynucleotide sequence, said modification configured to prevent fragments originat…
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- C12Q1/6855Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N15/1093Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9012184B2 cover?
- The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at thei…
- Who is the assignee on this patent?
- Rigatti Roberto, Gormley Niall Anthony, Bignell Helen Rachel, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6855. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).