PatentsDB

Use of template switching for DNA synthesis

US9012183B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9012183-B2
Application numberUS-201214000513-A
CountryUS
Kind codeB2
Filing dateFeb 23, 2012
Priority dateFeb 23, 2011
Publication dateApr 21, 2015
Grant dateApr 21, 2015

How to read this patent

A practical reading order for non-experts. Skip the full description unless you need deep technical detail.

  1. Title

    What the patent document calls the invention.

  2. Abstract

    A short plain-language summary of the technical disclosure.

  3. Assignees and inventors

    Who owns or filed the patent and who is credited as inventor.

  4. Key dates

    Filing, priority, publication, and grant dates set the timeline.

  5. First independent claim

    The legal scope of protection — read this for what is actually claimed.

  6. CPC / IPC classifications

    Technology tags used to group this patent with similar filings.

  7. Citations and related patents

    Prior art links and similar publications in this corpus.

Abstract

Official abstract text for this publication.

A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleotide from its 3′ end to provide a DNA copy polynucleotide. The DNA copy polynucleotide includes a complementary target DNA polynucleotide that is synthesized using the target polynucleotide as a template. Methods of adding nucleotides to the double stranded template/primer substrate are also described. The method can be used to facilitate detection, PCR amplification, cloning, and determination of RNA and DNA sequences.

First claim

Opening claim text (preview).

What is claimed is: 1. A method of preparing a DNA copy of a target polynucleotide using template switching, comprising: mixing at least one double stranded template/primer substrate, consisting of a DNA primer oligonucleotide annealed with a complementary oligonucleotide template strand, with at least one target polynucleotide in a reaction medium, and adding a suitable amount of a non-retroviral reverse transcriptase to the reaction medium to extend the DNA primer oligonucleot…

Assignees

Inventors

Classifications

Patent family

Related publications grouped by family.

View patent family 46721224

External sources

Next steps

Free tools are coming soon. Tell us what you want to track and we'll notify you.

Get alerts for this patentView on Google Patents

Request deeper analysis

Frequently asked questions

Answers are generated from the same data shown on this page.

What does patent US9012183B2 cover?
A method of preparing a DNA copy of a target polynucleotide using template switching is described. The method includes mixing a double stranded template/primer substrate made up of a DNA primer oligonucleotide associated with a complementary oligonucleotide template strand with a target polynucleotide in a reaction medium and adding a suitable amount of a non-retroviral reverse transcriptase to…
Who is the assignee on this patent?
Lambowitz Alan M, Mohr Sabine, White Travis B, and 2 more
What technology area does this patent fall under?
Primary CPC classification C12N15/1096. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).