Yeast display of proteins in the periplasmic space
US-2024102202-A1 · Mar 28, 2024 · US
Multi-chain eukaryotic display vectors and uses thereof
US9012181B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012181-B2 |
| Application number | US-62533709-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 24, 2009 |
| Priority date | Oct 1, 2001 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Abstract
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A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set). The use of such matched vector sets provides flexibility and versatility in the generation of eukaryotic display libraries, for example the ability to generate and to display multi-chain polypeptides by combining and recombining vectors that express variegations of the individual chains of a multi-chain polypeptide. Entire repertoires of novel chain combinations can be devised using such vector sets.
First claim
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What is claimed is: 1. A method for displaying a library of antibodies on the surface of yeast cells, comprising: providing yeast cells transformed with a library of yeast expression vectors encoding a library of antibodies each comprising an antibody heavy chain variable region (V H ) and an antibody light chain variable region (V L ), wherein at least one yeast expression vector comprises one or more homologous recombination sites and has undergone homologous recombination such…
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Chemistry & Metallurgy · mapped topic
- C12N15/1037Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9012181B2 cover?
- A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab frag…
- Who is the assignee on this patent?
- Hufton Simon E, Hoogenboom Hendricus R J M, Dyax Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1037. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).