TARGET-BASED METHOD FOR HIGH-THROUGHPUT AND SUBCLASS SPECIFIC IgG GLYCAN PROFILING IN HUMAN PLASMA
US-2024353417-A1 · Oct 24, 2024 · US
Dipeptides to enhance yield and viability from cell cultures
US9012178B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012178-B2 |
| Application number | US-201113814397-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 5, 2011 |
| Priority date | Aug 5, 2010 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Abstract
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The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.
First claim
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What is claimed is: 1. A method of culturing Chinese hamster ovary (CHO) cells that have been recombinantly engineered to express a protein, the method comprising growing the CHO cells in a serum-free medium during a growth phase, and growing the CHO cells in a serum-free defined production medium during a production phase, wherein during the production phase the serum-free medium is supplemented with at least one dipeptide selected from Tyr-His, Tyr-Lys, His-Gly, and Ala-His, and…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9012178B2 cover?
- The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.
- Who is the assignee on this patent?
- Kang Sohye, Deshpande Rohini, Mccoy Rebecca E, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification C07K16/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).