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Methods for detection and quantitation of small RNAs
US9012149B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9012149-B2 |
| Application number | US-201313745599-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 18, 2013 |
| Priority date | Nov 4, 2008 |
| Publication date | Apr 21, 2015 |
| Grant date | Apr 21, 2015 |
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Abstract
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Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse transcription reactions are described. Using these reaction conditions greater sensitivity and specificity of amplification of small RNAs including miRNAs is achieved.
First claim
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We claim: 1. A method for preparing a cDNA copy of a small RNA molecule, comprising: (a) providing a small RNA from a biological sample, wherein said RNA is from 18 to 28 nucleotides in length; (b) incubating the small RNA with an enzyme capable of catalyzing the addition of nucleotides at the 3′ end of the small RNA in the presence of a single ribonucleotide triphosphate selected from the group consisting of ATP, GTP, UTP, and CTP and at a final concentration of divalent magnes…
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- C12P19/34Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9012149B2 cover?
- Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse transcription reactions are described.…
- Who is the assignee on this patent?
- Sabiosciences Corp, Qiagen Sciences Llc
- What technology area does this patent fall under?
- Primary CPC classification C12P19/34. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).