DIG-3 insecticidal Cry toxins

Patent metadata

Field	Value
Publication number	US-9006520-B2
Application number	US-201213669130-A
Country	US
Kind code	B2
Filing date	Nov 5, 2012
Priority date	Apr 17, 2009
Publication date	Apr 14, 2015
Grant date	Apr 14, 2015

Abstract

DIG-3 Cry toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are useful to control insect pests.

First claim

The invention claimed is:

1. A transgenic plant cell comprising a nucleic acid molecule that encodes an isolated polypeptide comprising a core toxin segment having at least 97% sequence identity to the amino acid sequence of residues 73 to 643 of SEQ ID NO:2, wherein said polypeptide has insecticidal activity, and wherein said plant cell also comprises an insecticidal protein selected from the group consisting of a Cry1F protein and a Cry1A protein.

2. The plant cell of claim 1 , wherein said cell is selected from the group consisting of a corn plant cell and a vegetable plant cell.

3. A method of controlling a lepidopteran, said method comprising the steps of:
(i) producing a polypeptide comprising a core toxin segment having at least 97% sequence identity to the amino acid sequence of residues 73 to 643 of SEQ ID NO:2, wherein said polypeptide has insecticidal activity; and
(ii) providing the polypeptide produced in step (i) and at least one additional insecticidal protein to said lepidopteran,
wherein said additional insecticidal protein is selected from the group consisting of a Cry1F protein and a Cry1A protein, and wherein said lepidopteran is selected from the group consisting of a European corn borer that is resistant to Cry1F, and a diamond back moth that is resistant to a Cry1A.

4. The method of claim 3 , wherein said lepidopteran is said European corn borer, and said insecticidal protein is said Cry1F protein.

5. The method of claim 3 , wherein said lepidopteran is said diamond back moth, and said insecticidal protein is said Cry1A protein.

6. The method of claim 3 , where said method is used to control a pest population, wherein said pest population is selected from the group consisting of a population of uropean corn borer that is resistant to Cry1F, and a diamond back moth that is resistant to a Cry1A.

7. The method of claim 6 , wherein said population is said population of European corn borer, and said insecticidal protein is said Cry1F protein.

8. The method of claim 6 , wherein said population is said population of diamond back moth, and said insecticidal protein is said Cry1A protein.

9. A transgenic plant comprising a plurality of cells of claim 1 .

10. The plant cell of claim 1 , wherein said insecticidal protein is said Cry1F protein.

11. The plant cell of claim 1 , wherein said insecticidal protein is said Cry1A protein.

12. The plant cell of claim 11 , wherein said Cry1A protein is selected from the group consisting of a Cry1Ab protein and a Cry1Ac protein.

13. The method of claim 5 , wherein said Cry1A protein is selected from the group consisting of a Cry1Ab protein and a Cry1Ac protein.

14. The method of claim 8 , wherein said Cry1A protein selected from the group consisting of a Cry1Ab protein and a Cry1Ac protein.

Assignees

• Dow Agrosciences Llc

Inventors

• Lira Justin M
• Butler Holly Jean
• Smith Doug A
• Narva Kenneth
• Meade Thomas

Classifications

• C12N15/8286

  for insect resistance · CPC title

• A01N43/50 Primary

  1,3-Diazoles; Hydrogenated 1,3-diazoles · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title