Microfluidic interface for highly parallel addressing of sensing arrays

Patent metadata

Field	Value
Publication number	US-8999726-B2
Application number	US-201313776601-A
Country	US
Kind code	B2
Filing date	Feb 25, 2013
Priority date	Sep 30, 2005
Publication date	Apr 7, 2015
Grant date	Apr 7, 2015

Abstract

Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on, a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter. The spotter is particularly useful for depositing proteins in high concentrations on a surface, since the spotter may be placed on a surface for an extended period of time.

First claim

What is claimed is:

1. A method of depositing at least one substance on a surface, the method comprising:
loading a multiplicity of storage zones of a microspotter, via sample handling equipment, with the at least one substance;
placing a face of the microspotter in proximity to the surface sufficient to form a seal between the microspotter face and the surface, wherein the microspotter face includes orifices fluidicly connected to the multiplicity of storage zones;
flowing th…

Assignees

• Univ Utah Res Found

Inventors

• Chang-Yen David A
• Natarajan Sriram
• Eckman Josh
• Gale Bruce K
• Myszka David

Classifications

• B01L2300/0636

  Operations & Transport · mapped topic

• B01J19/0046 Primary

  Operations & Transport · mapped topic

• G01N2035/00158

  Physics · mapped topic

• B01L2300/0819

  Operations & Transport · mapped topic

• C12N15/1093

  Chemistry & Metallurgy · mapped topic