Methods and compounds for detection of molecular targets

Patent metadata

Field	Value
Publication number	US-8999639-B2
Application number	US-201313856569-A
Country	US
Kind code	B2
Filing date	Apr 4, 2013
Priority date	Feb 19, 2009
Publication date	Apr 7, 2015
Grant date	Apr 7, 2015

Abstract

The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.

First claim

The invention claimed is:

1. A method of detecting a target in a sample, comprising:
(i) incubating a target or a sample comprising thereof with one or more binding agents with peroxidase activity, wherein said one or more binding agents is capable of directly or indirectly binding to the target to form a complex comprising the target and the one or more binding agents;
(ii) incubating the complex of (i) in a water solution comprising:
1) 3,3′ diaminobenzidine,
2) a peroxide…

Assignees

• Dako Denmark As

Inventors

• Lohse Jesper

Classifications

• C08G69/40

  Chemistry & Metallurgy · mapped topic

• C12Q1/28

  Chemistry & Metallurgy · mapped topic

• C08G69/48

  Chemistry & Metallurgy · mapped topic

• G01N33/54393 Primary

  Physics · mapped topic

• C08G63/672 Primary

  Chemistry & Metallurgy · mapped topic