Using oligonucleotide microarrays to analyze genomic differences for the prediction of heterosis

Patent metadata

Field	Value
Publication number	US-8996318-B2
Application number	US-34488708-A
Country	US
Kind code	B2
Filing date	Dec 29, 2008
Priority date	Dec 28, 2007
Publication date	Mar 31, 2015
Grant date	Mar 31, 2015

Abstract

A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyzes of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

First claim

What is claimed is:

1. A method for predicting the heterotic phenotype of progeny plants with unknown heterotic phenotypes, the method comprising:
a) obtaining sample genomic DNA from a plurality of inbred parental plants from a plurality of crosses, wherein each of said crosses produces hybrid progeny plants possessing a known heterotic phenotype;
b) performing a comparative genomic hybridization technique on the sample genomic DNA to detect DNA copy number variations between g…

Assignees

• Beatty Mary
• Janni James A
• Lightner Jonathan E
• Rafalski J Antoni
• Pioneer Hi Bred Int
• Du Pont

Inventors

• Beatty Mary
• Janni James A
• Lightner Jonathan E
• Rafalski J Antoni

Classifications

• C12Q1/6809 Primary

  Chemistry & Metallurgy · mapped topic

• C12Q2539/101

  Chemistry & Metallurgy · mapped topic

• G16B20/10

  Physics · mapped topic

• C12Q1/6895 Primary

  Chemistry & Metallurgy · mapped topic

• C12Q2600/16

  Chemistry & Metallurgy · mapped topic