Volumetric Imaging
US-2024418652-A1 · Dec 19, 2024 · US
US8994807B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8994807-B2 |
| Application number | US-201013257588-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 18, 2010 |
| Priority date | Mar 18, 2009 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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A system ( 100 ) and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage ( 160 ). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera ( 155 ). Object planes in corresponding recorded regions of interest are recorded in the camera ( 155 ). A signal from the regions of interest is combined into a three dimensional image.
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What is claimed is: 1. A microscopy system for creating three dimensional images using probe molecules, comprising: a sample stage for mounting a sample, said sample containing the probe molecules; an optional activation light source configured to illuminate the sample with an activation light and to activate probe molecules in at least one subset of the probe molecules; a readout light source configured to illuminate the sample with a readout light and to cause luminescence i…
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