Microprojectile bombardment transformation of Brassica

Patent metadata

Field	Value
Publication number	US-8993845-B2
Application number	US-201414320685-A
Country	US
Kind code	B2
Filing date	Jul 1, 2014
Priority date	Nov 10, 2005
Publication date	Mar 31, 2015
Grant date	Mar 31, 2015

Abstract

The invention relates to the genetic manipulation of plants, particularly plants of the genus Brassica . Methods are provided for producing transgenic Brassica plants involving the introduction of a DNA construct by microprojectile bombardment into pre-incubated microspores, microspore-derived embryos and microspore-derived hypocotyls. The methods find use in the development of improved agricultural varieties of Brassica plants through the incorporation of desirable agronomic traits.

First claim

What is claimed is:

1. A method of producing a transformed Brassica cell by particle bombardment, comprising:
(a) Culturing an explant derived from a pre-incubated microspore, said explant comprising a cell, under a condition of plasmolysis for a period of about half an hour to about 4 hours prior to bombardment;
(b) introducing a DNA construct by microprojectile bombardment into an exposed cell on a surface of the explant, wherein the explant is under the condition of plasmol…

Assignees

• Pioneer Hi Bred Int

Inventors

• Chen Wenpin
• Tulsieram Lomas

Classifications

• C12N15/8207 Primary

  Chemistry & Metallurgy · mapped topic