Fatty acid elongation components and uses thereof

Patent metadata

Field	Value
Publication number	US-8993841-B2
Application number	US-201013376745-A
Country	US
Kind code	B2
Filing date	May 20, 2010
Priority date	Jun 8, 2009
Publication date	Mar 31, 2015
Grant date	Mar 31, 2015

Abstract

The invention provides isolated nucleic acid molecules which encodes a novel fatty acid nECR. The invention also provides recombinant expression vectors containing nECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA.

First claim

The invention claimed is:

1. A polynucleotide comprising an expression control sequence operatively linked to a heterologous nucleic acid sequence selected from the group consisting of:
a) the nucleic acid sequence of SEQ ID NO 1 or 3;
b) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or 4;
c) a nucleic acid sequence having at least 50% sequence identity to the nucleic acid sequence of a) or b), wherein said nucleic acid sequence encodes a polypeptide having fatty acid dehydratase/enoyl-CoA reductase (nECR) activity; and
d) a nucleic acid sequence encoding a polypeptide having nECR activity and comprising an amino acid sequence having at least 50% sequence identity to the amino acid sequence of SEQ ID NO 2 or 4.

2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises a terminator sequence operatively linked to the nucleic acid sequence.

3. A vector comprising the polynucleotide of claim 1 .

4. A host cell comprising the polynucleotide of claim 1 .

5. A method for the manufacture of a polypeptide comprisirig:
a) cultivating a host cell comprising the polynucleotide of claim 1 under conditions which allow for the production of a polypeptide encoded by said nucleic acid sequence; and
b) obtaining the polypeptide from the host cell of step a).

6. A non-human transgenic organism comprising the polynucleotide of claim 1 , wherein said organism is a microoraanism, yeast, or plant.

7. A plant, plant part, or plant seed comprising the polynucleotide of claim 1 .

8. A method for the manufacture of a polyunsaturated fatty acid comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell; and
b) obtaining said polyunsaturated fatty acids from said host cell.

9. The method of claim 8 , wherein said polyunsaturated fatty acid is arachidonic acid (ARA), eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA).

10. A method for the manufacture of an oil, lipid, or fatty acid composition comprising:
a) cultivating the host cell of claim 4 under conditions which allow for the production of polyunsaturated fatty acids in said host cell;
b) obtaining said polyunsaturated fatty acids from said host cell; and
c) formulating the polyunsaturated tatty acid as an oil, lipid, or fatty acid composition.

11. The method of claim 10 , wherein said oil, lipid, or fatty acid composition is used for feed, foodstuffs, cosmetics, or medicaments.

12. A method for the manufacture of a polyunsaturated fatty acid comprising:
a) cultivating the non-human transgenic organism of claim 6 under conditions which allow for the production of polyunsaturated fatty acids in said organism; and
b) obtaining said polyunsaturated fatty acids from said non-human transgenic organism.

13. The method of claim 12 , wherein the polyunsaturated fatty acid is arachidonic acid (ARA), eicosapentacnoic acid (EPA) or docosahexaenoic acid (DHA).

14. A method for the manufacture of an oil, lipid, or fatty acid composition comprising:
a) cultivating the non-human transgenic organism of claim 6 under conditions which allow for the production of polyunsaturated fatty acids in said organism;
b) obtaining said polyunsaturated fatty acids from said non-human transgenic organism;
c) formulating the polyunsaturated fatty acid as an oil, lipid, or fatty acid composition.

15. The method of claim 14 , wherein said oil, lipid, or fatter acid composition is used for feed, foodstuffs, cosmetics, or medicaments.

16. A polynucleotide comprising an expression control sequence operatively linked to a heterologous nucleic acid sequence selected from the group consisting of:
a) the nucleic acid sequence of SEQ ID NO: 1 or 3;
b) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or 4; and
c) a nucleic acid sequence encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2 or 4 and having nECR activity.

17. The polynucleotide of claim 16 , wherein said nucleic acid sequence encodes a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2 or 4 and having nECR activity.

Assignees

• Napier Johnathan A
• Sayanova Olga
• Beaudoin Frederic
• Basf Plant Science Co Gmbh

Inventors

• Napier Johnathan A
• Sayanova Olga
• Beaudoin Frederic

Classifications

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• C12N9/001

  acting on the CH-CH group of donors (1.3) · CPC title

• C12P7/6427

  Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone · CPC title

• C12P7/6472

  containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone · CPC title