Plant-based production of heterologous proteins

US8993839B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-8993839-B2
Application numberUS-201113186423-A
CountryUS
Kind codeB2
Filing dateJul 19, 2011
Priority dateJul 19, 2010
Publication dateMar 31, 2015
Grant dateMar 31, 2015

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Abstract

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Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium , allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.

First claim

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We claim: 1. A plant cell expressing a heterologous protein, the plant cell comprising a cucumber mosaic virus (CMV)-based viral amplicon, wherein the amplicon comprises multiple amplicon segments, capable of amplifying the transcription of a nucleic acid sequence encoding the heterologous protein, the amplicon comprising: a) a first amplicon segment comprising RNA 1 from CMV subgroup I, b) a second amplicon segment comprising RNA 2 from CMV subgroup I, and c) a third amplicon…

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What does patent US8993839B2 cover?
Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium , allowing expression of the heterologous protein, and recovering the heterologous prote…
Who is the assignee on this patent?
Hwang Minsook, Lindenmuth Benjamin E, Mcdonald Karen A, and 5 more
What technology area does this patent fall under?
Primary CPC classification C12N15/8257. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Mar 31 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).