Modified coronavirus structural protein
US-2024226271-A1 · Jul 11, 2024 · US
US8993839B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993839-B2 |
| Application number | US-201113186423-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 19, 2011 |
| Priority date | Jul 19, 2010 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium , allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.
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We claim: 1. A plant cell expressing a heterologous protein, the plant cell comprising a cucumber mosaic virus (CMV)-based viral amplicon, wherein the amplicon comprises multiple amplicon segments, capable of amplifying the transcription of a nucleic acid sequence encoding the heterologous protein, the amplicon comprising: a) a first amplicon segment comprising RNA 1 from CMV subgroup I, b) a second amplicon segment comprising RNA 2 from CMV subgroup I, and c) a third amplicon…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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