Method for stitching vehicle interior components and components formed from the method
US-9889802-B2 · Feb 13, 2018 · US
RNA interference mediating small RNA molecules
US8993745B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993745-B2 |
| Application number | US-87930010-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 10, 2010 |
| Priority date | Dec 1, 2000 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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Abstract
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Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3′ ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
First claim
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The invention claimed is: 1. A eukaryotic cell or a eukaryotic non-human organism comprising at least one double-stranded RNA molecule capable of mediating RNA interference, wherein: (i) each RNA strand of the RNA molecule independently consists of 19 to 25 nucleotides in length; (ii) at least one RNA strand of the RNA molecule forms a single-stranded 3′-overhang from 1 to 5 nucleotides; (iii) a strand of the RNA molecule is at least 85% identical to a target mRNA molecule in…
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- What does patent US8993745B2 cover?
- Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chem…
- Who is the assignee on this patent?
- Tuschl Thomas, Elbashir Sayda Mahgoub, Lendeckel Winfried, and 4 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/113. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 31 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).