Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Removal of PCR inhibitors
US8993341B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993341-B2 |
| Application number | US-201213469989-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 11, 2012 |
| Priority date | May 12, 2011 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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Abstract
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Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g, compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool samples, with insoluble polyvinylpyrrolidone (PVP) to form PVP-assay inhibitor complexes, and spin filtration to separate the PVP-assay inhibitor complexes from the crude sample preparations to produce clarified samples that exhibit reduced assay inhibition.
First claim
Opening claim text (preview).
We claim: 1. A method for removing an assay inhibitor from a crude sample preparation comprising a nucleic acid, the method comprising: a) adding insoluble polyvinylpyrrolidone particles having a diameter of 100 micrometers to 130 micrometers to said crude sample preparation prior to isolating the nucleic acid under conditions wherein said assay inhibitor binds to said polyvinylpyrrolidone to produce a complex, wherein said crude sample preparation is a supernatant prepared from a…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Cross-Sectional Technologies · mapped topic
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- What does patent US8993341B2 cover?
- Provided herein is technology relating to processing and preparing samples and particularly, but not exclusively, to methods, systems, and kits for removing assay inhibitors, e.g, compounds that inhibit polymerase chain reaction, from samples comprising nucleic acids. In particular, the technology is directed toward treating crude sample preparations, such as supernatants from homogenized stool…
- Who is the assignee on this patent?
- Bruinsma Janelle J, Shenoi Hemanth D, Domanico Michael J, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 31 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).