Delivery and formulation of engineered nucleic acids
US-2024252645-A1 · Aug 1, 2024 · US
Media conditioning for improving gene delivery efficiency to differentiating embryonic stem cells
US8993328B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993328-B2 |
| Application number | US-56490309-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 22, 2009 |
| Priority date | Sep 22, 2008 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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Abstract
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The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from ˜1.4% to ˜3.7% of the population.
First claim
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We claim: 1. A method for delivering a non-viral plasmid vector to a differentiating embryonic stem cell comprising: culturing a differentiating human or murine embryonic stem cell in a serum starved condition for about 1 to about 3 days, delivering to the cell cultured in the serum starved condition a non-viral plasmid vector encoding and capable of expressing a bioactive agent; and expressing the bioactive agent within the differentiating embryonic stem cell.…
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- C12N15/87Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8993328B2 cover?
- The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and inc…
- Who is the assignee on this patent?
- Yarmush Martin L, Wallenstein Eric J, Schloss Rene S, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/87. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 31 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).