Quantitative pcr method using internal control
US-2024368681-A1 · Nov 7, 2024 · US
Hot start reverse transcription by primer design
US8993240B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8993240-B2 |
| Application number | US-50463309-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 16, 2009 |
| Priority date | Jul 15, 2005 |
| Publication date | Mar 31, 2015 |
| Grant date | Mar 31, 2015 |
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Abstract
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The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.
First claim
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We claim: 1. A method of reducing primer extension products at a low temperature and allowing primer extension at a high temperature comprising; forming a reaction mixture at the low temperature below about 27° C., wherein the reaction mixture comprises a target polynucleotide, a reverse transcriptase, and a hot start primer, wherein the hot start primer comprises a loop and a self-complementary stem, wherein the self-complementary stem comprises a target-specific region, but said…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6848Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/6876Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US8993240B2 cover?
- The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.
- Who is the assignee on this patent?
- Lao Kai Qin, Straus Neil A, Livak Kenneth J, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6848. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 31 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).