Quantitative pcr method using internal control
US-2024368681-A1 · Nov 7, 2024 · US
Setting of multiple priming oligonucleotides for solid gel amplification in hydrogels
US8986959B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986959-B2 |
| Application number | US-201113819420-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 29, 2011 |
| Priority date | Aug 30, 2010 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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Abstract
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The present invention provides for a novel system and method for amplification and detection of nucleic acids within a microfluidic device wherein multiple nucleotides capable of priming PCR are present within the system and substantially sequestered within separate hydrogel posts therein.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for detecting a nucleic acid molecule within a hydrogel post array comprising a) Depositing on a substantially planar surface plural aqueous solutions each containing at least one nucleic acid capable of initiating an amplification of a selected template nucleic acid and a viscosity increasing solute, said plural aqueous solutions not being in fluid communication with each other and forming a multiplicity of deposits; b) Allowing said aqueous solu…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/686Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6844Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8986959B2 cover?
- The present invention provides for a novel system and method for amplification and detection of nucleic acids within a microfluidic device wherein multiple nucleotides capable of priming PCR are present within the system and substantially sequestered within separate hydrogel posts therein.
- Who is the assignee on this patent?
- Atrazhev Alexey, Univ Alberta
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/686. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).