Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Methods for generating target specific probes for solution based capture
US8986958B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986958-B2 |
| Application number | US-201013260906-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 30, 2010 |
| Priority date | Mar 30, 2009 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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Abstract
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Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5′ region having a first restriction enzyme recognition sequence and flanked by a 3′ region having a second restriction enzyme recognition sequence, and a region which hybridizes to a capture nucleic acid molecule. The single-stranded nucleic acid probes are useful for solution-based capture methods.
First claim
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What is claimed: 1. A method for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleotide sequence, the method comprising: a) providing a starting population of linear double-stranded nucleic acid precursor molecules each precursor molecule having (i) a probe region having the predetermined sequence which is flanked at a 5′ and a 3′ end by a first and a second restriction enzyme recognition sequence for generating ligation su…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6811Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8986958B2 cover?
- Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5′ region having a first restriction enzyme recognition sequence and flanked by a 3′ region having a second restriction enzyme…
- Who is the assignee on this patent?
- Raymond Christopher, Life Technologies Corp
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6811. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).