Biocatalytic oxidation process with AlkL gene product
US-9200043-B2 · Dec 1, 2015 · US
US8986950B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986950-B2 |
| Application number | US-201113205288-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 8, 2011 |
| Priority date | Jun 19, 2003 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.
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The invention claimed is: 1. A microbial cell for higher production of vanillin, said cell comprising at least the following three heterologous enzymatic activities: i) a 3-dehydroshikimate dehydratase ii) an aromatic carboxylic acid reductase; and iii) a 3-O-methyl transferase; wherein said cell has been transformed with an expression vector comprising a heterologous nucleic acid encoding an UDPG-glycosyltransferase capable of glycosylating vanillin.…
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