Pathogen specific nucleic acid fragment and application thereof
US-2024352539-A1 · Oct 24, 2024 · US
Real-time method for the detection of viable micro-organisms
US8986946B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986946-B2 |
| Application number | US-80956608-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 19, 2008 |
| Priority date | Dec 20, 2007 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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Abstract
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The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for real-time detection of viable living cells comprising a. adding a cell-permeable, phototautomeric compound to a medium to be tested for the presence or absence of living cells, in which medium the phototautomeric compound does not emit fluorescence; wherein the fluorescence of said phototautomeric compound is high at an intracellular pH, as compared to low emission at the pH of the medium in which said phototautomeric compound is supplie…
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Physics · mapped topic
- C12Q1/04Primary
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
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- What does patent US8986946B2 cover?
- The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, th…
- Who is the assignee on this patent?
- Kort Remco, Schuren Frank Henri Johan, Montijn Roy Christiaan, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/04. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).