Methods and compositions related to cooperative primers and reverse transcription
US-2024182992-A1 · Jun 6, 2024 · US
US8986933B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986933-B2 |
| Application number | US-31575808-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 5, 2008 |
| Priority date | Dec 5, 2008 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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A process for detecting human rhinovirus nucleic acid in a biological sample, includes producing an amplification product by amplifying an human bocavirus nucleotide sequence using a forward primer of SEQ ID NO: 1, and a reverse primer of SEQ ID NO: 2, and measuring said amplification product to detect human rhinovirus in said biological sample. Also provided are reagents and methods for detecting and distinguishing human rhinovirus from other viruses. A kit is provided for detecting and quantifying human rhinovirus in a biological sample.
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The invention claimed is: 1. A process of detecting human rhinovirus in a biological sample comprising: producing an amplification product by amplifying a human rhinovirus nucleotide sequence using a forward primer homologous to a region within 356-563 of human rhinovirus, and a reverse primer homologous to a region within 356-563 of human rhinovirus, under conditions suitable for a polymerase chain reaction; and measuring said amplification product to detect human rhinovirus in sa…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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