Compositions and methods for accurately identifying mutations
US-2024409996-A1 · Dec 12, 2024 · US
Nanopore sequencing using charge blockade labels
US8986932B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986932-B2 |
| Application number | US-201414317451-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 27, 2014 |
| Priority date | Apr 10, 2009 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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Abstract
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The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of the nucleotide analog such that the charge blockade labels are cleaved when the nucleotide analog is incorporated into a growing nucleic acid and the charge blockade label is detected by the nanopore to determine the presence and identity of the incorporated nucleotide and thereby determine the sequence of a template nucleic acid.
First claim
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We claim: 1. A composition comprising: a plurality of polyrnerase enzymes, each complexed with a template nucleic acid, each polymerase enzyme attached to a nanopore or attached proximal to a nanopore, and nucleic acid sequencing reagents including at least one nucleotide analog having the structure NS-PP-L-B wherein NS comprise a nucleoside moiety, PP comprises a polyphosphate chain with at least two phosphates, L comprises a linker, and B comprises a blockade label.…
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Physics · mapped topic
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
Physics · mapped topic
- C12Q1/6869Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8986932B2 cover?
- The invention relates to devices and methods for nanopore sequencing. The invention includes compositions and methods of nucleic acid sequencing using a single polymerase enzyme complex comprising a polymerase enzyme and a template nucleic acid attached proximal to a nanopore, and nucleotide analogs in solution comprising charge blockade label that are attached to the polyphosphate portion of t…
- Who is the assignee on this patent?
- Pacific Biosciences California
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6869. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).