Compositions and methods for accurately identifying mutations
US-2024409996-A1 · Dec 12, 2024 · US
Sequencing reactions with alkali metal cations for pulse width control
US8986930B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8986930-B2 |
| Application number | US-201113177775-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 7, 2011 |
| Priority date | Jul 12, 2010 |
| Publication date | Mar 24, 2015 |
| Grant date | Mar 24, 2015 |
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Abstract
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Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.
First claim
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We claim: 1. A composition for single molecule nucleic acid sequencing comprising: a modified recombinant phi-29 type DNA polymerase enzyme having at least 80% sequence similarity to SEQ ID NO: 1, a primer, a template nucleic acid, and a plurality of labeled nucleotide analogs wherein the composition comprises a mixture of alkali metals comprising K+ at a concentration of from about 50 mM to about 300 mM, and Li+ at a concentration of from about 0.05 mM and about 40 mM.…
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- C12Q1/6869Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US8986930B2 cover?
- Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequenc…
- Who is the assignee on this patent?
- Fedorov Andrei, Lyle John, Bjornson Keith, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6869. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).