Methods for rapid separation and purification of dna topological forms
US-2024218352-A1 · Jul 4, 2024 · US
Method for isolating nucleic acids
US8980552B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8980552-B2 |
| Application number | US-201013378466-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 21, 2010 |
| Priority date | Jun 18, 2009 |
| Publication date | Mar 17, 2015 |
| Grant date | Mar 17, 2015 |
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Abstract
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The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of: (a) binding the nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding support material in the presence of at least one chaotropic compound, at least two different detergents and at least one branched and/or unbranched alcohol, preferably isopropanol, with the concentration of said alcohol being 40% (v/v); (b) optionally eluting the bound nucleic acids from the nucleic acid-binding support material. The method of the invention is particularly suitable for purifying circulating, extracellular miRNA from blood.
First claim
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The invention claimed is: 1. A method of isolating and/or purifying nucleic acids from a nucleic acid-containing starting material, comprising: (a) binding the nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding support material in the presence of at least one chaotropic compound, at least one ionic detergent, at least one nonionic detergent, and at least one branched and/or unbranched alcohol, wherein the con…
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- C12N15/1006Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US8980552B2 cover?
- The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of: (a) binding the nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding …
- Who is the assignee on this patent?
- Horlitz Martin, Sprenger-Haussels Markus, Qiagen Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1006. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 17 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).