Extraction and diagnostic fluid devices, systems and methods of use

Patent metadata

Field	Value
Publication number	US-8979770-B2
Application number	US-28050407-A
Country	US
Kind code	B2
Filing date	Feb 15, 2007
Priority date	Feb 24, 2006
Publication date	Mar 17, 2015
Grant date	Mar 17, 2015

Abstract

The present invention provides devices, systems and methods for RNA isolation from biological samples containing white blood cells, such as whole blood. The devices have a device body that includes a first chamber having a first membrane that selectively binds white blood cells, a second chamber having a second membrane that reversibly binds RNA and a plurality of ports that are removably attached to a reagent pack and waste receptacle via a valving system controlled by a control unit.

First claim

The embodiments of the invention in which an exclusive property or privilege is claimed are as defined as follows:

1. A device for isolating RNA from a sample containing white blood cells, the device comprising:
a device body that includes:
an inlet port for receiving a sample containing white blood cells;
a first chamber that includes a first membrane that binds white blood cells;
a second chamber that includes a second membrane, wherein the second membrane is a glass-fiber…

Assignees

• Fare Thomas L
• Kessler Deborah A
• Kilian Kristopher A
• Merck Sharp & Dohme

Inventors

• Fare Thomas L
• Kessler Deborah A
• Kilian Kristopher A

Classifications

• C12N15/1017 Primary

  Chemistry & Metallurgy · mapped topic