Endoscopic imaging apparatus and method
US-12133634-B2 · Nov 5, 2024 · US
US8977331B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8977331-B2 |
| Application number | US-201213713745-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 13, 2012 |
| Priority date | Dec 13, 2012 |
| Publication date | Mar 10, 2015 |
| Grant date | Mar 10, 2015 |
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Systems and methods for imaging are presented. The method includes producing excitation light configured to induce fluorescence in an imaging agent that selectively binds to a target species in a region of interest (ROI) of a subject that also includes a background species. A first and a second spectral region are selected such that a determined difference between fluorescence corresponding to the target and the background species in the first spectral region differs from a corresponding difference in the second spectral region. First and second fluorescence images are generated from the fluorescence corresponding to the first and second spectral regions. Additionally, a fluorescence ratio for the background species in the first and second fluorescence image is determined. The first fluorescence image is then multiplied or divided with the determined ratio to generate an intermediate image that is subtracted from the second fluorescent image to reconstruct a background-subtracted image.
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The invention claimed is: 1. An imaging system, comprising: one or more illumination sources configured to produce excitation light that induces fluorescence emissions in an imaging agent configured to bind to a target species in a region of interest of a subject, wherein the region of interest comprises the target species and a background species; at least one optical element configured to separate the fluorescence emissions into a first spectral region and a second spectral re…
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