Compositions and methods for altering alpha- and beta-tocotrienol content using multiple transgenes

Patent metadata

Field	Value
Publication number	US-8975472-B2
Application number	US-201313733203-A
Country	US
Kind code	B2
Filing date	Jan 3, 2013
Priority date	Oct 4, 2007
Publication date	Mar 10, 2015
Grant date	Mar 10, 2015

Abstract

Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter the content of alpha-tocotrienol, beta-tocotrienol, or both, in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids.

First claim

What is claimed is:

1. A method of increasing the level of alpha tocotrienol, beta-tocotrienol, or both, in a microbial cell, comprising:
stably incorporating into the genome of a microbial cell:
(a) a first recombinant nucleic acid molecule comprising at least one regulatory sequence operably linked to at least one nucleotide sequence selected from the group consisting of:
(i) the nucleotide sequence set forth in SEQ ID NO:15;
(ii) a nucleotide sequence encoding the amino ac…

Assignees

• Du Pont

Inventors

• Meyer Knut

Classifications

• C12N15/8243

  Chemistry & Metallurgy · mapped topic

• C12N15/79 Primary

  Chemistry & Metallurgy · mapped topic

• A61K31/355 Primary

  Human Necessities · mapped topic

• C12N9/1007

  Chemistry & Metallurgy · mapped topic