Polymer particles, nucleic acid polymer particles and methods of making and using the same

Patent metadata

Field	Value
Publication number	US-8975302-B2
Application number	US-201213543642-A
Country	US
Kind code	B2
Filing date	Jul 6, 2012
Priority date	Jul 7, 2011
Publication date	Mar 10, 2015
Grant date	Mar 10, 2015

Abstract

The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. The disclosure also relates to methods of making nucleic acid polymer particles having the same method steps and wherein the aqueous gel reaction mixture includes a nucleic acid fragment, such as a primer.

First claim

What is claimed is:

1. A method of making polymer particles, said method comprising:
making an aqueous gel reaction mixture including an acrylamide or derivative thereof monomer and including a nucleic acid fragment;
forming an emulsion comprising dispersed aqueous phase micelles of gel reaction mixture in a continuous phase;
adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and
performing a polymerization reaction in the micel…

Assignees

• Light David
• Rosenblum Barnett
• Life Technologies Corp

Inventors

• Light David
• Rosenblum Barnett

Classifications

• C12Q1/6834 Primary

  Chemistry & Metallurgy · mapped topic

• C08F251/00

  Chemistry & Metallurgy · mapped topic

• C08F222/385

  Chemistry & Metallurgy · mapped topic

• C12Q1/6869 Primary

  Chemistry & Metallurgy · mapped topic

• C08F2/32

  Chemistry & Metallurgy · mapped topic