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Deducing exon connectivity by RNA-templated DNA ligation/sequencing
US8975019B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8975019-B2 |
| Application number | US-90667810-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 18, 2010 |
| Priority date | Oct 19, 2009 |
| Publication date | Mar 10, 2015 |
| Grant date | Mar 10, 2015 |
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Abstract
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A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide.
First claim
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We claim: 1. A method, comprising: a) providing: i) a plurality of ligamer subsets, wherein each of said ligamer subsets comprise a plurality of different complementary regions, wherein at least two of said plurality of different complementarity regions are separated by a central region, wherein said central region has a different exon-specific barcode sequence, wherein each said different barcode sequence comprises a different identifier code for a different specific exon, where…
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- C12N15/1065Primary
Chemistry & Metallurgy · mapped topic
- C12N15/1031Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US8975019B2 cover?
- A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides a…
- Who is the assignee on this patent?
- Zamore Phillip D, Moore Melissa J, Roy Christian, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1065. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 10 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).