Alkynyl-derivatized cap analogs, preparation and uses thereof

Patent metadata

Field	Value
Publication number	US-8969545-B2
Application number	US-201213655103-A
Country	US
Kind code	B2
Filing date	Oct 18, 2012
Priority date	Oct 18, 2011
Publication date	Mar 3, 2015
Grant date	Mar 3, 2015

Abstract

Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

First claim

What is claimed is:

1. A composition comprising an alkynyl-derivatized cap analog having the structure:
R 3 7,3′-O-alkynyl G[5′]p[p]np[5′]G,
R 3 7,3′-O-alkynyl G[5′]p[p]np[5′]A,
R 3 7,2′-O-alkynyl G[ 5 ′]p[p]np[5′]G,
R 3 7,2′-O-alkynyl G[ 5 ′]p[p]np[5′]A,
or a salt thereof,
wherein
R 3 is alkyl or arylalkyl,
the alkynyl moiety comprises 3-24 carbon atoms, a terminal alkyne, and is optionally substituted,
n is 1, 2, or 3,
A is adenosine, and
G is guanosin…

Assignees

• Life Technologies Corp

Inventors

• Kore Anilkumar R
• Muthian Shanmugasundaram
• Gee Kyle

Classifications

• C07H1/02

  Chemistry & Metallurgy · mapped topic

• C07H1/00

  Chemistry & Metallurgy · mapped topic

• C07H1/04

  Chemistry & Metallurgy · mapped topic

• C12P19/34

  Chemistry & Metallurgy · mapped topic

• C12N2770/36171

  Chemistry & Metallurgy · mapped topic