Method for producing hematopoietic stem cells using pyrazole compounds
US-9212348-B2 · Dec 15, 2015 · US
US8969079B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8969079-B2 |
| Application number | US-201113819409-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 8, 2011 |
| Priority date | Aug 27, 2010 |
| Publication date | Mar 3, 2015 |
| Grant date | Mar 3, 2015 |
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The present invention relates to a technique of using monocytic blood cells to effectively culture and proliferate blood adult stem cells and progenitor cells that only exist in small quantities to effectively obtain large quantities of stem cells. According to the present invention, the limitation of being able to derive only small quantities of stem cells from blood can be overcome, and the pluripotency of stem cells can easily be obtained.
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We claim: 1. A method for proliferating blood adult stem cells using human blood-born hematospheres, comprising: isolating mononuclear cells from blood; aggregate culturing the isolated cells in a three-dimensional structure; proliferating the three-dimensionally cultured cells to form blood-born hematospheres; and dissociating the blood-born hematospheres into single cells; wherein the blood adult stem cells are contained and proliferated within the blood-born hematospher…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Human Necessities · mapped topic
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