Method and agents for detecting luciferase activity
US-2024018566-A1 · Jan 18, 2024 · US
Method for detecting pyrophosphate by means of bioluminescence detection
US8969045B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8969045-B2 |
| Application number | US-51521807-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 7, 2007 |
| Priority date | Nov 20, 2006 |
| Publication date | Mar 3, 2015 |
| Grant date | Mar 3, 2015 |
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Abstract
Official abstract text for this publication.
The invention relates to methods for detecting pyrophosphate by means of bioluminescence detection. In addition, methods for measuring chemical, especially enzyme-catalyzed, reactions in which pyrophosphate is formed or consumed are described. Such reactions are catalyzed for example by guanylyl cyclases, adenylyl cyclases, DNA polymerases or RNA polymerases. The novel methods are distinguished by high sensitivity and low susceptibility to interference, can easily be automated and miniaturized and are additionally suitable for carrying out continuous measurements. The methods can be employed particularly advantageously in the area of medical diagnosis and biomedical research, including pharmaceutical active ingredient research.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for detecting pyrophosphate, comprising the steps of: (a) incubating a composition consisting essentially of dehydroluciferin, ATP, and luciferase which is activated by pyrophosphate, to make an incubated composition, the ATP being present in an amount that is in excess of the dehydroluciferin and luciferase, (b) combining the incubated composition with luciferin to make a detection mix, the luciferin being present in an amount that is in ex…
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- C12Q1/66Primary
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
Physics · mapped topic
Physics · mapped topic
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- What does patent US8969045B2 cover?
- The invention relates to methods for detecting pyrophosphate by means of bioluminescence detection. In addition, methods for measuring chemical, especially enzyme-catalyzed, reactions in which pyrophosphate is formed or consumed are described. Such reactions are catalyzed for example by guanylyl cyclases, adenylyl cyclases, DNA polymerases or RNA polymerases. The novel methods are disting…
- Who is the assignee on this patent?
- Burkhardt Nils, Heitmeier Stefan, Bayer Ip Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/66. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 03 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).