Combination of fluorescent dyes for the detection of nucleic acids

Patent metadata

Field	Value
Publication number	US-8962820-B2
Application number	US-201314091804-A
Country	US
Kind code	B2
Filing date	Nov 27, 2013
Priority date	Dec 1, 2008
Publication date	Feb 24, 2015
Grant date	Feb 24, 2015

Abstract

The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.

First claim

The invention claimed is:

1. A composition comprising:
(i) a first nucleic acid to which a blue florescent dye is covalently attached;
(ii) a second nucleic acid to which a green fluorescent dye is covalently attached;
(iii) a third nucleic acid to which a yellow fluorescent dye is covalently attached, wherein the yellow florescent dye has a relative detection sensitivity in an emission spectrum (peak area of the yellow fluorescent dye:peak area of the blue fluorescent dye) of…

Assignees

• Qiagen Gmbh

Inventors

• Brabetz Werner
• Weber Cornelia

Classifications

• C12Q1/686 Primary

  Chemistry & Metallurgy · mapped topic

• C12Q1/6846

  Chemistry & Metallurgy · mapped topic