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Growth hormone and interferon-alpha 2 glycoproteins produced in plants
US8962811B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8962811-B2 |
| Application number | US-201313855946-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 3, 2013 |
| Priority date | Jan 14, 2004 |
| Publication date | Feb 24, 2015 |
| Grant date | Feb 24, 2015 |
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Abstract
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Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
First claim
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What is claimed is: 1. A plant-derived biologically active fusion glycoprotein, comprising: a) at least one plant hydroxyproline O-glycosylation glycomodule covalently linked to b) the C-terminus of a biologically active mammalian protein; wherein the at least one plant hydroxyproline O-glycosylation glycomodule is (X-Hyp) n (SEQ ID NO: 3), where n is 2-25 and X is serine, alanine, threonine, or valine; and wherein the biologically active fusion glycoprotein exhibits a pharmaco…
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- What does patent US8962811B2 cover?
- Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in incre…
- Who is the assignee on this patent?
- Univ Ohio
- What technology area does this patent fall under?
- Primary CPC classification C07K14/415. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).