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Method of characterizing glycans attached to glycoproteins
US8962345B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8962345-B2 |
| Application number | US-201113112822-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 20, 2011 |
| Priority date | May 21, 2010 |
| Publication date | Feb 24, 2015 |
| Grant date | Feb 24, 2015 |
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Abstract
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A method of characterizing glycans attached to glycoproteins is disclosed herein. The method comprises a first step of immobilizing the glycoproteins on colloidal particles forming glycoprotein/colloidal particles. The glycans on the glycoproteins may then be characterized, for example the composition and/or structure of glycans may be characterized or the glycans attached to proteins may be identified. Characterization may be accomplished by either binding the glycoprotein/colloidal particles with one or more binding agents and assessing the aggregation of the glycoprotein/colloidal particles or by cleaving glycans from the glycoprotein/colloidal particles with a cleaving agent and analyzing the glycans.
First claim
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The invention claimed is: 1. A method of characterizing glycans attached to glycoproteins comprising the steps of: a) spontaneously immobilizing said glycans attached to glycoproteins on colloidal particles forming glycoprotein/colloidal particles; b) optically assessing the glycoprotein/colloidal particles or measuring their size; c) aggregating said glycoprotein/colloidal particles with a binding agent forming an aggregate; d) optically assessing the aggregate or measuring…
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Physics · mapped topic
- G01N33/6854Primary
Physics · mapped topic
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- What does patent US8962345B2 cover?
- A method of characterizing glycans attached to glycoproteins is disclosed herein. The method comprises a first step of immobilizing the glycoproteins on colloidal particles forming glycoprotein/colloidal particles. The glycans on the glycoproteins may then be characterized, for example the composition and/or structure of glycans may be characterized or the glycans attached to proteins may be id…
- Who is the assignee on this patent?
- Zangmeister Rebecca A, Sanchez-Pomales Germarie, Morris Todd A, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification G01N33/6854. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Feb 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).