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Increasing cell culture population doublings for long-term growth of finite life span human cell cultures
US8962325B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8962325-B2 |
| Application number | US-24247408-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 30, 2008 |
| Priority date | Mar 31, 2006 |
| Publication date | Feb 24, 2015 |
| Grant date | Feb 24, 2015 |
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Abstract
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Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
First claim
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What is claimed is: 1. A method of increasing pre-stasis population doublings in a cell culture of human epithelial cells with finite life span, comprising the steps of: a) providing a cell culture medium comprising a mixture of i) MM medium with serum and ii) a different, serum-free medium, with defined ingredients for growth of human mammary epithelial cells, wherein MM medium and the serum-free medium are optimized for a human mammary epithelial cell, and wherein the mixture…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N5/0631Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US8962325B2 cover?
- Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with an…
- Who is the assignee on this patent?
- Stampfer Martha R, Garbe James C, Univ California
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0631. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).