Transformed euglena and process for producing same
US-2015368655-A1 · Dec 24, 2015 · US
Methods for selecting eukaryotic cells expressing a heterologous protein
US8962274B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8962274-B2 |
| Application number | US-201013203546-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 26, 2010 |
| Priority date | Feb 27, 2009 |
| Publication date | Feb 24, 2015 |
| Grant date | Feb 24, 2015 |
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Abstract
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The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprising (a) providing a plurality of eukaryotic host cells, wherein cellular viability of said host cells is dependent upon folate uptake, wherein said host cells comprise at least (i) a foreign polynucleotide encoding a product of interest and (ii) a foreign polynucleotide encoding a DHFR enzyme; (b) culturing said plurality of eukaryotic host cells in a selective culture medium comprising at least an inhibitor of DHFR and folate in a limiting concentration; and (c) selecting at least one eukaryotic host cell expressing the product of interest. Also provided is a method for expressing a product of interest which is based on host cells selected by said method and a cell culture medium.
First claim
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The invention claimed is: 1. A method for producing a product of interest, comprising at least the following steps: (a) providing a plurality of eukaryotic host cells, wherein cellular viability of said host cells is dependent upon folate uptake, wherein said eukaryotic host cells comprise at least (i) an introduced polynucleotide encoding a product of interest and (ii) an introduced polynucleotide encoding a DHFR enzyme; (b) culturing said plurality of eukaryotic host cell…
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- What does patent US8962274B2 cover?
- The invention pertains to a method for selecting at least one eukaryotic host cell expressing a product of interest, comprising (a) providing a plurality of eukaryotic host cells, wherein cellular viability of said host cells is dependent upon folate uptake, wherein said host cells comprise at least (i) a foreign polynucleotide encoding a product of interest and (ii) a for…
- Who is the assignee on this patent?
- Jostock Thomas, Knopf Hans-Peter, Novartis Ag
- What technology area does this patent fall under?
- Primary CPC classification C12N15/79. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 24 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).