Fructosyl peptidyl oxidase

Patent metadata

Field	Value
Publication number	US-8962271-B2
Application number	US-201414227693-A
Country	US
Kind code	B2
Filing date	Mar 27, 2014
Priority date	Aug 3, 2009
Publication date	Feb 24, 2015
Grant date	Feb 24, 2015

Abstract

In one form, a fructosyl peptidyl oxidase derived from a budding yeast Phaeosphaeria nodorum for assaying a glycated protein in a sample is provided. The fructosyl peptidyl oxidase has higher activity toward fructosyl valine as well as fructosyl valyl histidine, and may be useful in assaying HbA1c with higher sensitivity and specificity. Still, other forms include unique methods, techniques, systems and devices involving a fructosyl peptidyl oxidase.

First claim

What is claimed is:

1. A method for assaying a glycated protein in a sample, comprising:
contacting the sample with one of:
a fructosyl peptidyl oxidase comprising an amino acid sequence having at least 70% identity with the amino acid sequence set forth in SEQ ID NO:1 and a Km value toward fructosyl valyl histidine of 1 mM or less;
a fructosyl peptidyl oxidase comprising an amino acid sequence having at least 70% identity with the amino acid sequence set forth in SEQ ID NO:1…

Assignees

• Roche Diagnostics Operations
• Ultizyme International Ltd
• Ultizyme International Ltd

Inventors

• Ikebukuro Kazunori
• Koji Sode

Classifications

• C12Q1/004

  Chemistry & Metallurgy · mapped topic

• G01N33/723

  Physics · mapped topic

• C12Q1/26 Primary

  Chemistry & Metallurgy · mapped topic