Methods and nucleic acid molecules for aav vector selection
US-2024417717-A1 · Dec 19, 2024 · US
US8961995B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-8961995-B2 |
| Application number | US-201313789215-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 7, 2013 |
| Priority date | Sep 20, 2012 |
| Publication date | Feb 24, 2015 |
| Grant date | Feb 24, 2015 |
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The present invention provides alphavirus replicons and methods of their use in producing heterologous protein.
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What is claimed is: 1. A recombinant replicon nucleic acid comprising: a) a nucleic acid sequence encoding a first alphavirus 5′ untranslated region (5′ UTR) and a 51 nucleotide conserved sequence element (51 -nt CSE); b) a nucleic acid sequence encoding alphavirus nonstructural proteins nsP1, nsP2, nsP3 and nsP4; c) a nucleic acid sequence encoding an alphavirus subgenornic promoter; d) a nucleic acid sequence encoding a second alphavirus 5′ untranslated region (5′ UTR) and…
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