Integrated sample preparation systems and stabilized enzyme mixtures

Patent metadata

Field	Value
Publication number	US-8961899-B2
Application number	US-201313925355-A
Country	US
Kind code	B2
Filing date	Jun 24, 2013
Priority date	May 6, 2010
Publication date	Feb 24, 2015
Grant date	Feb 24, 2015

Abstract

The present invention provides integrated sample preparation systems and stabilized enzyme mixtures. In particular, the present invention provides microfluidic cards configured for processing a sample and generating DNA libraries that are suitable for use in sequencing methods (e.g., next generation sequencing methods) or other suitable nucleic acid analysis methods. The present invention also provides stabilized enzyme mixtures containing an enzyme (e.g., an enzyme used in whole genome amplification), BSA, and a sugar. Such enzyme mixtures may be lyophilized and stored at room temperature without significant loss of enzyme activity for months.

First claim

We claim:

1. A microfluidic card, comprising:
a) a loading port configured for introduction of a biological sample;
b) a nucleic acid extraction sub-circuit wherein said nucleic acid extraction sub-circuit comprises:
i) a nucleic acid extraction component configured to bind nucleic acids present in said biological sample;
ii) a wash buffer;
iii) an elution buffer; and
iv) a component configured for moving said elution buffer over said nucleic acid extraction component to…

Assignees

• Ibis Biosciences Inc

Inventors

• Eshoo Mark
• Picuri John

Classifications

• C12Q1/6806 Primary

  Chemistry & Metallurgy · mapped topic

• B01L3/502753

  Operations & Transport · mapped topic

• B01L3/5027 Primary

  Operations & Transport · mapped topic

• B01L2300/087

  Operations & Transport · mapped topic

• B01L2300/0867

  Operations & Transport · mapped topic